Fee Schedule, Current as of Aug 1, 2016
A. Transmission Electron Microscopy
|Routine Specimen Preparation:*|
|1) fixation through embedding||1-2 samples||$375.00|
|over 10 samples||+$50.00/each additional sample|
|1) thick sectioning & staining/blk||$40.00/blk|
|2) thin sectioning & staining/blk||(for every 4 grids)||$60.00/blk|
|per hour spent on scope||$165.00||$80.00|
*Fifteen mls of a modified Karnovsky's fixative can be provided for up to 10 specimens, additional fixatives or special types will be at an extra cost. Tissue specimens for TEM should be about 2 x 2 x 2mm or smaller. For special types of specimens (cultured cells, thin layers of tissue, tissue that requires special orientation) contact Caroline Miller (firstname.lastname@example.org) for instructions.
**This represents a reasonable amount of supplies for fixation and embedding, including, slides, grids, stains, and equipment usage. The EM Center provides 4 hours of training in electron microscopy techniques and use of the TEM. The cost for this training is charged to the researcher at the rate of $165/hour. Final decision on whether researcher can go on their own will be determined by the lab manager.
***This represents direct digital images. EM film is not used in the EM Center
B. Special Procedures, Immunocytochemistry-EM level (no independent)
|1) fixation-embedding, Unicryl||1-2 samples||$375.00|
|over 10 samples||+$50.00/sample|
|2) Cryo Preparation||Needs to be arranged|
|1) Thick sectioning & staining/blk||
|2) Thin sectioning & staining/blk||8 Grids||$65.00|
Eight grids represents enough for 2 immuno attempts, there will be a $5 charge per grid over eight.
|3) Cryo sectioning/blk, thick and thin||$50.00/hr|
There will be an additional charge for liquid Nitrogen ($4/liter), a minimum of 40 liters.
|4) Immunostaining at the TEM level, for each attempt & antibody****||$450.00|
5) Immunostaining at the LM level (on plastic thick sections), for each attempt & antibody****
|6) Negative Staining (per grid)||$45.00|
****Results cannot be guaranteed since antibody specificity/titer, quality/fixation of tissue and amount of target protein can limit success. Investigator provides primary antibody and necessary background literature.
C. Scanning Electron Microscopy:
|Routine Specimen Preparation*|
|1) Fixation and Coating||5 samples||$500.00|
|over 5 samples||+$45.00/each additional sample|
|2) Coating Only||$20.00/Sample|
|Per hour spent on scope||$165.00||$80.00|
**This represents a reasonable amount of supplies for fixation through drying (CPD or chemical), mounting and coating. The EM Center provides 4 hours of training in electron microscopy techniques and use of the SEM. The cost of this training is charged to the researcher at the rate of $165/hour. Final decision on whether researcher can go on their own will be determined by the lab manager.
D. EM evaluation/consultation:
Each incoming project receives an hour consultation at no charge, thereafter there is an $85 per hour charge for time spent researching techniques, evaluating data (quantitative and/or qualitative) or for anything beyond routine. The EM Center provides 4 hours of training in electron microscopy techniques or use of either the TEM or SEM. The cost for this training is charged to the investigator at the rate of $165/hour. If retraining is necessary, that is charged at the rate of $165.00/hr.
E. Outside/ Commercial Projects
Each project will be charged double the cost of regular fee schedule, due to lack of subsidy.