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Services/Equipment

The Indiana University School of Medicine Electron Microscopy Center is a full service research laboratory providing both Transmission and Scanning Electron Microscopy. The center can provide the technical services to help design and then implement experiments needing either type of microscopy. Free consultation with the manager or director is provided with any new experiment. The service provided can apply both traditional methods and more recent technical developments to suit the investigator's needs. The services that are available are as follows:

Transmission Electron Microscopy (TEM)

  1. Standard routine processing of specimens with fixation through embedding.
  2. Standard thick and thin sectioning with staining.
  3. Viewing and photography on the TEM.
  4. Standard photography, digital images provided on CD.
  5. Stereo pairs on 200nm sections (specimen permitting)
  6. Standard supplies (including some fixatives) are provided.
  7. Standard routine processing includes either tissue or cell cultures either as monolayers, on membranes or as pellets and special orientation. The facility has the capability to do negative staining and rotary shadowing.

These procedures are provided as a full service. If an investigator has documented training in EM techniques, knowledge and ability for correct use of the equipment, then the EM Center allows for independent work at a lower cost (see fee schedule). Training will be provided at the full service price until confirmation of capabilities on the equipment. The EM Center Director has the authority to revoke the independent use privilege or require retraining to ensure the safety of the EM Center equipment. All additional training requested or required are billed at the technician rate.

Immunocytochemistry (TEM, full service only)

  1. Standard routine processing of specimens with fixation and embedding in Unicryl
  2. Standard routine thick and thin sectioning for post-embedding immunostaining
  3. Immunostaining*
  4. Viewing and photography on the TEM
  5. Digital images provided on CD

*Primary antibody provided by the investigator, EM Center provides the secondary, can do pre-embedding immunostaining if desired.

Cryo Fixation/Processing and Sectioning (TEM, full service only)

  1. High pressure freezing (HPF) of tissue specimens or cell cultures
  2. Processing of HPF specimens through embedding using automated freeze substitution
  3. Processing of specimens for Cryo sectioning
  4. Standard routine thick and thin sectioning of embedded specimens
  5. Thick and thin Cryo sectioning of specimens
  6. Immunostaining*
  7. Viewing and photography on TEM
  8. Digital images provided on CD

*Primary antibody provided by the investigator, EM Center provides the secondary, can do pre-embedding immunostaining if desired

Scanning Electron Microscopy (SEM)

  1. Standard routine processing of specimens with fixation, critical point or chemical drying and mounting
  2. Viewing and photography on the SEM
  3. Standard supplies (including some fixatives) are provided
  4. Digital images provided on CD
  5. Standard routine processing includes either tissue or cell cultures either as monolayers

These procedures are provided as a full service. If an investigator has documented training in EM techniques, knowledge and ability for correct use of the equipment, then the EM Center allows for independent work at a lower cost (see fee schedule). Training will be provided at the full service price until confirmation of capabilities on the equipment. The EM Center Director has the authority to revoke the independent use privilege or require retraining to ensure the safety of the EM Center equipment. All additional training requested or required are billed at the technician rate.

Equipment Available in the EM Center

TEM Microscope (picture)

  • Tecnai G2 12 Bio Twin
  • FEI, Hillsboro, OR equipped with AMT CCD Camera (Advanced Microscopy Techniques, Danvers, MA)

SEM Microscope (picture)

  • JEOL 6390 LV (Peabody MA)

High Pressure Freezer and Automatic Freezer Substitution

  • High Pressure Freezer (picture) (Leica, Bannockburn, IL)

Ultramicrotomes

  • UCT Ultracut with FCS
  • FCS, Cryosectioning Attachment (picture) (Leica, Bannockburn, IL)

Critical Point Dryer

  • Samdri-790 (Tousimis, Rockville, MD)

Sputter Coater

  • Polaron (Energy Beam Sciences, East Gramby, CT)

Vibratome

  • Warner Instruments (Harvard Bioscience Company, Series 1000)

Policies

Submitting Material

Prospective users of the EM Center can make an inquiry about future projects, either by email, phone, or in person. The EM Center's fee schedule is located on our website. We encourage clients to check it out and set up a time to discuss the project in person with the EM Center prior to providing specimens. We ask that you come prepared with pertinent information relating to your particular project. At that time a request for specimen submission, including an estimate of costs for the project can be completed so clients and the EM Center personnel are thoroughly familiar with the proposed project.

This center's primary goal si to generate high quality data using electron microscopy. Specimen quality is essential to this process, without properly fixed and prepared specimens it is impossible to generate good and interpretable images. It is this center's policy to review all protocols for incoming specimens and we reserve the right to either reject specimens and/or suggest necessary changes to aid in obtaining adequate and quality results.

Data Analysis and Transfer

All data and results belong to the customer. Neither will be shared outside this center without the researcher's consent.

Confidentiality

All data and results generated can only be accessed by the researcher, the EM Center Director and technical staff.

Conflict Resolution

The EM Center Director will discuss all issues with the investigator to help resolve the problem. If the issue cannot be resolved, it will be submitted to the EM Center's advisory committee.

Cost Recovery

The fee schedule was set up to offset the substantial cost of maintaining this center without making our services cost prohibitive. Any changes in the service requested by the client may result in additional costs above the estimate provided at the initial review of the proposed project and estimate.

Prioritization

An accession number is given to all incoming projects (specimens). Unless discussed and an arrangement made beforehand, all projects are on a first-come first-serve basis. IU projects have priority over externally submitted projects.

Publication/Authorship Policy

The Electron Microscopy Center provides electron microscopy services on a fee-for-service basis. Basic processing and microscopy are the responsibility of EM Center personnel. But interpretations of resulting data are the responsibility of the submitting investigator. The EM Center should be acknowledged in publications in which microscopy results are utilized. Co authorship on resulting publications should be considered when projects brought to the EM Center require significant additional scientific or intellectual input from the EM Center Director or personnel.

Check the fee schedule for current charges on services.

Please do not hesitate in contacting the center either by phone at (317) 274-8730 or email if you have any questions regarding electron microscopy.

Dept. of Pathology & Laboratory Medicine Administration Office | Van Nuys Medical Science Building | 635 Barnhill Drive, room A-128 | Indianapolis, IN 46202 Indiana University Health Pathology Laboratory: 350 W. 11th Street, Indianapolis, Indiana 46202